Effects of Antioxidant Activity in the Platelet Rich Plasma


Wienaldi1), I Nyoman Ehrich Lister2), Bungaran3)

 

1)Doctoral Program, Faculty of Medicine, University of Prima Indonesia Medan

2)Department of Biochemistry and Molecular Biology, Faculty of Medicine,

University of Prima Indonesia Medan

3)Department of Urology, Faculty of Medicine, Prima Indonesia University, Medan

 

ABSTRACT

 

Background: Antioxidant compounds are increasingly being used along with the development of public understanding of their role in inhibiting various diseases. Plate­let Rich Plasma (PRP) is known to have the ability to fight free radicals and reactive oxygen species (ROS), increase antioxidants and have anti-inflammatory effects. PRP is an effective alternative treatment method of choice at this time and continues to develop. In recent years, PRP has developed very promising and safe to use, so that more and more people are using this method for treatment. This study aimed to determine the difference between the antioxidant activity of PRP and the IC50 value of its antioxidant activity.

Subjects and Method: This was a experimental study. PRP was made by centrifuging rat blood at 1,500 rpm at 20°C for 10 minutes. The plasma fraction separated from the red blood cells was taken and centrifuged again at 2,000 rpm at 20°C for 15 minutes. The plasma fraction obtained was standardized and the antioxidant activity was tested using the 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) method.

Results: The antioxidant activity of PRP in ABTS increased with decreasing extract concentration. At the lowest concentration (6.25 g/mL), PRP showed the highest anti­oxidant activity against ABTS trapping with an activity percentage of 5.85%. At the lowest concentration (6.25 g/mL), PRP showed the highest antioxidant activity against ABTS trapping with an IC50 average of 126.60 g/mL.

Conclusion: The antioxidant activity of PRP) in ABTS increases with the concen­tration of the extract decreases. It is indicated that PRP has antioxidant activity on ABTS radicals.

Keywords: platelet rich plasma, ABTS, antioxidant

Correspondence: Wienaldi. Faculty of Medicine, Prima Indonesia University, Medan, North Sumatera, Indonesia. Email: dr.wienaldi@gmail.com. Mobile: +62 85270130535.

DOI: https://doi.org/10.26911/AB.Medicine.ICPH.08.2021.42

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