The Effect of Red Spinach (Amaranthus Tricolor L.) Leaf Ethanol Extract Concentration on the Physical Quality of Face Serum and Antioxidant Activity using 2,2-Diphenyl-1-Picryl-Hydrazyl-Hydrate (Dpph) Method


Kharisma Aprilita Rosyidah, Riana Putri Rahmawati, Muhammad Nurul Fadel, Hasriyani

 

Study Program of Pharmacy, Universitas Muhammadiyah Kudus, Central Java

 

ABSTRACT

Background: Damage to the skin due to free radicals will interfere with the appear­ance so that the skin needs to be protected. Red spinach contains flavonoids which can be used as a source of antioxidants to inhibit free radicals. Antioxidant effect for facial skin care would be better formulated in a topical form. One form of cosmetic dosage form that has developed at this time is serum. Serum is a preparation with a high concentration of active ingredients and low viscosity, which delivers a thin film of the active ingredient on the skin surface. This study aimed to determine the effect of the concentration of ethanolic extract of red spinach leaves (Amaranthus tricolor L.) on the physical quality of facial serum and antioxidant potential using the DPPH method.

Subjects and Method: This study was a laboratory experiment with variations in the concentration of ethanolic extract of red spinach leaves by 0%, 0.5%, 1%, and 1.5%. Physical quality tests carried out were organoleptic tests, homogeneity, pH, adhesion, dispersibility, viscosity, and stability of serum preparations for 14 days. Antioxidant potential test was assessed using 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) method with analysis using UV-VIS spectrophotometer.

Results: Serum preparation with 0.5% red spinach leaf ethanol extract concentration (Formula 1) had the most optimal physical quality because the resulting serum gave a clearer color and lower viscosity. Serum preparation with a concentration of 1.5% red spinach leaf ethanol extract (Formula 3) has the most optimal antioxidant potential with an IC50 value of 81.08 ppm which is a very strong antioxidant.

Conclusion: Formula 1 has the most optimal physical quality. Formula 3 has the most optimal antioxidant potential.

Keywords: red spinach, flavonoids, antioxidants.

Correspondence:Kharisma Aprilita Rosyidah. Study Program of Pharmacy, Universitas Muhammadiyah Kudus. Jl. Ganesha Raya No. 1, Purwosari, Kudus 59316, Central Java. Email: kharismaaprilita@umkudus.ac.id. Mobile: +6281393979092.

DOI: https://doi.org/10.26911/ICPHmedicine.FP.08.2021.07

 

Share this :

View PDF